One row was removed because the ref bases do not match the reference genome. +------------+------------+-----------+------+------+-----------+ | chromosome | start_hg19 | stop_hg19 | ref | alt | sample_id | +------------+------------+-----------+------+------+-----------+ | 19 | 27368 | 27369 | AC | A | 13861.p1 | +------------+------------+-----------+------+------+-----------+