Variant data was taken from the supplemental table 1.

Subjects that were negative or not recorded (NR) for Autism spectrum disorder or autistic behavior (HP:0000729) were excluded.

Subjects without cDNA change information were excluded. Transvar was used to determine genomic coordinates based on cDNA coordinates. Transcript used: ENST00000399982 (NM_001170629.1)

Subject 93 was excluded due to ambiguous cDNA change.