Variants found in unaffected family members were filtered out
LiftGenome was used to convert genome coordinates to the GRCh37/hg19 human genome assembly
All coordinates needed to be shifted 1 nt to the right to match up with the reference alleles - UCSC Genome Browser was used to confirm this
VARICARTA_phenotype column was removed as all individuals have ASD phenotype (recorded in rawvariant tab)